CLONES

Background

Bacterial E. coli clones are widely used to isolate, sequence and characterise subsets of DNA and RNA sequences, and are especially useful for characterising complex genomes such as Brassica. A range of different classes of clones have been used to characterise Expressed Sequence Tages (ESTs), as well as for shotgun Genome Survey Sequencing (GSS) of Brassica. Longer insert clones, particularly those in Bacterial Artificial Chromosomes (BACs) have been an essential platform for large-scale genomic mapping and the ongoing sequencing project.

Information for this page is based on the information we have to date - please provide any updates by sending to Graham King

• BACs
• ESTs
• GSS

BAC clone summary

 

Other BAC resources:

BACMan database (Plant Genome Mapping Lab at the University of Georgia )
JIC Genome Lab

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EST clone summary

(this table currently being updated, Jan 2009)